سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۲

نویسنده(ها):

M Amiri – National Institute for Genetic Engineering and Biotechnology(NRCGEB),Tehran,Iran & Khatam Institue of Higher Education
M Jalalirad – National Institute for Genetic Engineering and Biotechnology(NRCGEB),Tehran,Iran
M Houshmand – National Institute for Genetic Engineering and Biotechnology(NRCGEB),Tehran,Iran
R Mirfakhraie – National Institute for Genetic Engineering and Biotechnology(NRCGEB),Tehran,Iran & Azad Medical university

چکیده:

Cystic fibrosis (CF) is caused by mutations in the CF transmembrane conductance regulator (CFTR) gene and characteristically leads to prominent Lung and pancreatic malfunctions. A total of seventy two unrelated Iranian CF families were screened for the presence of mutations occurring in exons 4,7,10,11,13,20,21 and flanking intronic sequences. For mutation detection, PCR-SSCP analysis and sequencing were used.Keyword: cystic fibrosis; CFTR; SSCP; Sequencing