سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۴
Soheila Sepahdoost – Payam Noor University
Haleh Haghighi – Payam Noor University
Haleh Hashemi Sohi – National Research Center for Genetic Engineering and Biotechnology
Esmat Jourabchi – National Research Center for Genetic Engineering and Biotechnology
Using agro-infiltration, we have transiently expressed human Growth Hormone (hGH) in Medicago sativa and Trifolium alexanderium leaves. The presence of hGH was detected in leaves extracts by western blot and ELISA. The plant leaf types were effective factor in expression level of produced recombinant protein. Medicago leaves infiltrated for 30 minutes could be good candidate for this production system, but the expression rate in trifolium levaes was lower in 25 minutes that was the best time. .It could be discussed for the hydrolytic or toxic compounds that could be find in trifolium leaves.