دانلود مقاله Allele frequency distribution of D17S5 and D1S80 in Fars, Turk and Kurd ethic group of Iranian population
سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۳
Mohammad M Banoei – National Institute for Genetic Engineering and Biotechnology
Behnaz Bayat – National Institute for Genetic Engineering and Biotechnology
Mohammad M Sanati – National Institute for Genetic Engineering and Biotechnology
DNA samples were obtained from, Azeri and Kurd from unrelated individuals living in their main settlement of Iran. All of case who participated in this investigation were male and between the ages 25-55 years. Donors were checked for healthy to document in questionnaire with consent from. Polymerase Chaine Reaction (PCR) was performed for D17S5 and D1S80 with specificprimers. Amplified fragment were stained by 6% polyacrylamide gel electrophoresis using silver staining. We screened a total 150 individuals DNA (300 chromosome) 12 alleles and 16 alleles found at the D 17s5 and at the D1s80 markers respectively. D17S5 VNTR marker showed difference and was polymorphic in Fars in somparison with two ethnics group and same for D1S80 VNTR marker in Azeri. Statistical test would be discussed.