سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۴

نویسنده(ها):

S Zamanian – Department of Plant Pathology, College of Agricultural Sciences, Bahonar University of Kerman.
G.H Shahidi Bonjar – Department of Plant Pathology, College of Agricultural Sciences, Bahonar University of Kerman.
S Aghighi – Department of Plant Pathology, College of Agricultural Sciences, Bahonar University of Kerman,
M Kalantarzadeh – Department of Plant Pathology, College of Agricultural Sciences, Bahonar University of Kerman,

چکیده:

(Erwinia carotovora subsp. carotovora), (Ralstonia solanacearum) and (Streptomyces scabies) are destructive plant pathogens with worldwide economical importance. Chemical measures have lost their attractiveness because of development of resistant strains of pathogens and undesirable effects on environment. Biological control of plant pathogens has gained great importance worldwide. Actinomycetes, by virtue of their wide distribution and filamentous growth in soil, colonize the root surface and the rhizosphere and exert inhibitory effect on pathogenic microorganisms via production of biologically active secondary-metabolites. In order to obtain antibacterial antagonists from soil Actinomycetes, a vast survey was performed. Over 200 isolates of soil Actinomycetes were isolated and screened among which three isolate showed high level of activity in Agar disk and Well diffusion methods against (E. carotovora subsp. carotovora), (R. solanacearum) and (Streptomyces scabies). Active isolates were identified as: (Streptomyces coralus strain 63), (S. plicatus strain 101) and (S. olivaceus strain 102). High concentration of antibacterial agent was detected in 10 –۱۱th day in shake cultures. Longevity in vitro of active crude of (S. plicatus strain 101) against (E. carotovora subsp. carotovora) in soluble state determined about 30 days at room temperature. In thermal inactivation point studies, active crude retained activity up to 135° C. Antibacteral genes from theses strains may be proper candidates for genetic engineering of plants for increased tolerance against the tested cosmopolitan and highly destructive bacteria.