سال انتشار: ۱۳۸۷

محل انتشار: دومین کنگره بین المللی علوم و فناوری نانو

تعداد صفحات: ۲

نویسنده(ها):

S Atashpaz – Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences
A Barzegari – Agriculture Biotechnology Research Institute for Northwest & West of Iran,Tabriz, Iran
R Azarbaijani – Higher Education Complex of Agricultural and Natural Resource Sciences, University of Mazandaran, Sari

چکیده:

Isolation of genomic DNA is the first and the most important requirement in carrying out a genetic analysis, such as mutation detection or linkage analysis. This step is so important in molecular diagnosis that the success of the subsequent analysis depends on the isolation of DNA fragments that have good purity, integrity, and concentration [1].A large variety of DNA isolation methods have been developed for variety of bacterial species. Most of them proved to be reproducible and yielded sufficiently good quality DNA for PCR; however, their throughput was usually low, whereas their cost was not always acceptable. Moreover the usual protocols for DNA extraction remain time-consuming, slow and even hazardous [2,3,4]. Thus; in this study we compared four specific DNA extraction methods on their related bacterial samples with the presented method in this study