دانلود مقاله Comparison the prevalence of Listria monocytogenes in alimentary tract of ordinary carp and phytophagous fish in Tehran & Gilan provinces
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
M.H Ghafourian – Faculty of Veterinary Medicine, Islamic Azad University of Garmsar
M.H Zakeri – Faculty of Veterinary Medicine, Islamic Azad University of Garmsar
H Zakeri – Faculty of Veterinary Medicine, Islamic Azad University of Garmsar
D Zakeri – Private section veterinarian
Method & Materials: 120 samples of fish were taken from 2 hatchery pools in Gilan and Tehran. The culture medias were: 1) Brain and heart infusion broth, 2) listeria enrichment broth base, 3) listeria selective agar, 4) Nalidixic acid, 5) Kalium thiocyanat. We blend the alimentary canal of ordinary caro and phytophagous fish in a blender that include 225 ml of brain and heart infusion broth. Then 1ml of this mixture transferred to the tubes which have 9ml of listeria enrichment broth base. 0.1ml of listeria enrichment broth base transferred on listeria selective agar. other tests were: superficial tests, biochemical and subtraction.
Results & Conclusion: Only 4 samples (6.6%) were geram positive in term of Listria monocytogen serotype 4/b. The amount of contamination in the studied fish was the same, 2 cases in carp fish and 2 cases in phytophagous. The contaminated samples were only in Gilan Province and the reason might be high pollution of pisciculture pools in Gilan Province and /or low temperature microbial in this province in comparison to Tehran Province.