دانلود مقاله Delay in flowering and increase in biomass of transgenic tobacco (Nicotiana tabacum L.) expressing the Arabidopsis floral repressor gene FLC (FLOWERING LOCUS C)
سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۵
H Salehi – Department of Horticultural Science, College of Agriculture, Shiraz University.
C.B Ransom –
H.F Oraby –
Z Saddighi –
FLOWERING LOCUS C (FLC), a gene from Arabidopsis thaliana that acts as a flowering repressor, was expressed in tobacco (Nicotiana tabacum L. ‘Samsun’). Agrobacterium strain GV 3101 containing the FLC and bar selectable marker cassettes was employed for transformation. Five putative transgenic lines, resistant to 5 mg/L glufosinate ammonium, were selected and examined for the presence of FLC. Genomic DNA and total RNA were isolated from the leaves and used for polymerase chain reaction (PCR) and Northern blot analysis, respectively. Both DNA and RNA tests confirmed the integration and transcription of FLC in all five lines and their T1 progenies. Transgenic plants in one line showed an average of 36 d delay in flowering time compared to control plants, and the overall mean for all lines was 14 d. Transgenic plants also displayed increased leaf size and biomass yield and reduced height at flowering time.