سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۳

نویسنده(ها):

Rezvan Bagheri – Biotechnology Research Center, Pasteur institute, Tehran, Iran
Ramin Sarrami-Frooshani –
Hossein Khanahmad –
Farzaneh Sabahi –

چکیده:

An enzyme-linked oligosorbent assay (ELOSA) was developed for the detection on microtiter plates of PCR amplified human immunodeficiency DNA. In this nonisotopic PCR assay designated PCR-ELOSA, fragment of the HIV gag gene from peripheral blood mononuclear cells (PBMCs) was first amplified with a digoxigenin-labeled amplified DNA segment was reacted in solution with a probe labeled with biotin. Hybrids were captured in microtiter plate coated with streptavidin. Specific bound hybrids were detected by the addition of on enzyme-labeled antibody against digoxigenin and chromogenic substrate. This method was developed to increase both sensitivity of detection of PCR product.