سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۴
Navid Dinparast Djadid – Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran
Hesam Barjesteh – Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran
Flora Forouzesh –
Sedigheh Zakeri –
Interest in insect GSTs has primarily focused on their role in insecticide resistance. In this study, after WHO routine susceptibility test, DNA extracted from specimens of Anopheles stephensi collected from Kazeroon district in Fars province as control area and Saravan, Chabahar, Nikshahr districts in Sistan & Baluchistan province (under insecticide spraying area), followed by amplification of GSTe2 gene including exon I and II and full sequence of intron I. The sizes of amplified fragments are 492 and 489bp in An. stephensi specimens from control and under insecticide spraying areas. These fragments were purified and sequenced from both ends. The comparison of GSTe2 gene in Kazeroon and Nikshahr populations with other populations of Sistan & Baluchistan province (Saravan and Chabahar) showed 98% and 97% similarity, and also identified some nucleotide substitutions within these strains which none of them cause change in amino acid sequence. Comparison of nucleotide sequence of GSTe2 in An. stephensi populations with the main world malaria vector, An. gambiae revealed 86% homology, while amino acid similarity between two species is about 90%.