دانلود مقاله Effect of methisoprinol on the fish rhabdoviruses (VHSV, IHNV) and herpesvirus (CyHV-3) replications and protection against viral diseases: in vitro and in vivo study in rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio)
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
Andrzej K Siwicki – Department Microbiology and Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Poland
Barbara Kazuń – Department Fish Pathology and Immunology, IFI in Olsztyn, Poland
Edward Głąbski – Department Fish Pathology and Immunology, IFI in Olsztyn, Poland
Krzysztof Kazuń – Department Fish Pathology and Immunology, IFI in Olsztyn, Poland
Method & Materials: Monolayers of cell cultures were exposed to various doses of g/ml). The cell culture containingμmethisoprinol (0, 100, 200, 400, 600 and 800 methisoprinol were followed by infection with VHSV, IHNV or CyHV-3 suspension containing 107 TCID50 /ml. At 24 h, 48 h and 72 h after infection, cell cultures were submitted to an one-hour starvation period by replacing the medium with pre-heated PBS followed by a Ci/ml of [ 3H]-uridine or thymidine (Amersham, UK)μtwo-hour incubation with 10 and the counts per minute (cpm) evaluated in a scintillation counter (Beckman LS).
Results & Conclusion: The cytopathic effects were observed in virus-infected cultures without methisoprinol (control) at 24 h after infection with CyHV-3, VHSV and IHNV. The highest percent of inhibition 72 h after g/ml (90 %), compared to theμinfection was observed at doses of 400 and 600 lowest concentration of methisoprinol where only 25 % inhibition was observed. The present study demonstrates that methisoprinol inhibits the VHSV, IHNV from rainbow trout and CyHV-3 isolated from carp. The in vivo study showed that methisoprinol significantly stimulated nonspecific cellular and humoral defence mechanisms and protection against diseases in rainbow trout and carp.