سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۶
M Motahari – Biology Department, Faculty of Sciences, Shahid Bahonar University of Kerman, Iran
A Namaki shoshtari –
KH.M Kalantari – International Centre of Sciences and High Technology and Environmental Sciences, Mahan, Kerman
Effects of salinity (sodium chloride ) on growth and biological nitrogen fixation (BNF) was investigated in roots of alfalfa (Bami and American cultivars) inoculated by six different strains of Sinorhizobium bacteria.These strains included a wild type named S.meliloti wild type ( S), two mutated strains S.meliloti nifK-(S) and392259S. meliloti nifH-( S237), which were without plasmid and three strains W.T( pSRK9) , nifK-(pSRK9) and nifH-(pSRK9), with plasmid. (pSRK9) plasmid carried nitrogen fixation system of Agrobacterium tumefaciens. Salinity treatments were 0, 50, 100 mM sodium chloride. Results showed that dry weight production in those plants which were treated with 0,50 mM sodium chloride were the same. Mean comparison for active nodule number, dry weight of whole plant, sugar content of root and nitrogenase activity showed that both cultivars of alfalfa inoculated with nifK-(pSRK9) strain was much higher than others. In the other hand inoculation of alfalfa plants with high salinity tolerant of Sinorhizobium meliloti bacteria which were transferred with the plasmid contain the genes that are able to fix nitrogen can improve plant production under salinity.