سال انتشار: ۱۳۹۳
محل انتشار: اولین کنگره بین المللی و سیزدهمین کنگره ژنتیک ایران
تعداد صفحات: ۱
نویسنده(ها):
Shiva Mohammadi Moghanjoghi – MsC Iranian Biological Resource Center
Seyyed Abolhasan Shahzadeh Fazeli – (Ph.D)Iranian Biological Resource Center
Masome Asadi – (MsC)Iranian Biological Resource Center
Mehrnaz Izadpanah – (MsC)Iranian Biological Resource Center

چکیده:

Introduction: Xeroderma pigmentosum (XP) is a rare autosomal recessive disorder and has been found in all continents and racial groups. The symptoms are extreme sensitivity to sunlight, resulting in sunburn, pigment changes in the skin and a greatly elevated incidence of skin cancers. EBV induced immortalized Lymphoblastoid cells of this disease have been established. Purpose: There is essential need for infinite source of human cells and biological molecules for researcher all over thecountry especially biological source of rare disease like XP to genetic screening studies, genotype-phenotype correlationstudies, variety of molecular and functional assays along with immunological and cellular studies. Iranian Biologicalresource Center (IBRC) have established immoratalization of B cells of Xeroderma pigmentozum patient as a source for different medical studies. Method: B lymphocytes of the patient introduced to Epstein Barr virus and within 4 weeks these cells transformed to Lymphoblastoid cells (LCL) observed by clump formation in B cells. The EBV-producing marmoset B-cell line (B95-8) was used for source of EBV. Inter species identification of the cells was confirmed by multiplex PCR.Results: LCLs showed rosette morphology with doubling time of approximately 24 h. Transformation of peripheral Blymphocytes by Epstein- Barr virus (EBV) is the method of choice for generating lymphoblastoid cell lines (LCLs). Thismethod has been in use for the last two decades with a high success rate. With a somatic mutation rate of 0.3% and ease of cell maintenance, lymphoblastoid cells are still the preferred choice of storage for patient’s genetic material with minimal genetic and phenotypic aberrations