دانلود مقاله Evaluating the effects of orally administered propolis ethanolic extract on non-specific immunity in rainbow trout (Oncorhynchus mykiss), using neutrophil glass-adherent nitroblue tetrazolium (NBT) assay
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
O Beyraghdar – Fisheries Division, Natural Resources Department, Isfahan University of Technology
E Ebrahimi –
N Mahboobi-Soofiani –
A Samie –
(Oncorhynchus mykiss) in Iranian aquaculture and the proven immunostimulatory effects of propolis in mammals, this study was conducted to evaluate the effects of orally administered propolis ethanolic extract on the non-specific immunity in rainbow trout using neutrophil glass-adherent nitroblue tetrazolium (NBT) assay.
Method & Materials: Propolis was dissolved in ethanol and added to diets. Juvenile rainbow trout (mean initial weight 14 g) were fed on experimental diets (3% of biomass per day) supplemented with 0 (control), 0.5, 1.5, 4.5 and 9 g propolis/Kg diet for 8 weeks (four replicate tanks for each treatment). At the end of the experiment, blood samples were collected from caudal vein (four fish per replicate), and neutrophil
glass-adherent NBT assay was used to determine the respiratory burst activity. After adherence and staining procedures, the dark-blue staining cells were counted under microscope as NBT-positive cells.
Results & Conclusion: The number of NBT-positive cells (in fifty microliters of blood) for the control fish was 6.63±۰٫۴۸, and 6.75±۱٫۱۹, ۸٫۵۰±۲٫۱۲, ۸٫۵۰±۱٫۶۸ and 15.50±۲٫۳۴ in the groups receiving 0.5, 1.5, 4.5 and 9 g propolis/Kg diet, respectively. The present study showed that the administration of 9 g propolis/Kg diet resulted in a significant increase in the number of glass-adherent NBT-positive blood neutrophils of experimental fish after 8 weeks (P < 0.01). Evaluation of neutrophil function is valuable for assessment of the health and immune status of fish and the increased number of NBT-positive cells in this study can suggest that propolis constituents may be effective in immunopotentiating but the use of propolis as a fish immunostimulant for fish farming purposes needs validation with further studies on other non-specific immunity indicators, using different sources and doses, different routs of administration and other fish species.