دانلود مقاله Evaluation of arabinoxylo-oligosaccharides (AXOS) as a dietary supplement on immune responses of Siberian sturgeon (Acipenser baerii)
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
Z Geraylou – Laboratory of Aquatic Ecology and Evolutionary Biology, KU.Leuven, Belgium.
E Rurangwa – Laboratory of Aquatic Ecology and Evolutionary Biology, KU.Leuven, Belgium
C.M Courtin – Laboratory of Food Chemistry and Biochemistry & Leuven Food Science and Nutrition Research Centre (LFoRCe), KU.Leuven, Belgium
J.A Delcour – Laboratory of Food Chemistry and Biochemistry & Leuven Food Science and Nutrition Research Centre (LFoRCe), KU.Leuven, Belgium
Method & Materials: A basic diet for sturgeon was supplemented with 2% short AXOS 3-0.25 or long AXOS 32-0.30. Two hundred eighty eight juveniles of Siberian sturgeon, Acipenser baerii, weighing 25.9±۰٫۹ g were randomly distributed in 24 aquaria, with 8 replicate aquaria containing 12 fish each per treatment. Fish were fed diets at 3% of their body weight and sampled every 2 weeks to measure different zootechnical parameters. After 12 weeks, 8 fish from different experimental groups were randomly selected to study the effects of dietary AXOS on immune responses. The cellular and humoral immune responses measured included: phagocytic activity, oxidative burst respiratory assay (NBT), alternative haemolytic complement activity, lysozyme activity, peroxidase content, total immunoglobulin and bacterial agglutination.
Results & Conclusion: AXOS supplementation positively affected the growth of Siberian sturgeon, as the average weight of fish fed short and long AXOS was respectively 2.8% and 12.4% higher than that of control fish at harvest. However, due to strong individual variation, those differences in growth were not significant (p=0.286). The present work illustrated that AXOS modulate some cellular and humoral immune parameters of this fish species in a positive manner. Phagocytic activity, leucocyte peroxidase content and the haemolytic complement activity increased significantly as a result of prebiotic administration whereas there was no significant effect on respiratory burst, lysozyme activity, total immunoglobulin and specific antibody production against Vibrio proteolyticus. In conclusion, it is proven that short and long AXOS improve zootechnical performance and enhance cellular and humoral immune responses in A. baerii.