سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۴

نویسنده(ها):

Sadeq Vallian – Division of Genetics, Department of Biology, Faculty of Isfahan, The university of Isfahan
Hassan Moeini –

چکیده:

Genotyping for four short tandem repeat (STR) loci HUMvWA, HUMFES, HUMTPO, and HUMTH01 was examined in 110 unrelated individuals from the population of Isfahan province of IR Iran. The loci were genotyped using the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. The data demonstrated that the tetranucleotide repeat markers were all highly informative in the population examined. The STR markers were also found to have a relatively high degree of heterozygosity. Forensic and paternity indices including power of discrimination and exclusion as well as polymorphism information content and typical paternity index were determined for the STR alleles examined. Therefore, the STR loci studied have proven useful for paternity testing and individual identification in Iranian population, and the data could be used in construction of a genetic database.