سال انتشار: ۱۳۸۵

محل انتشار: یازدهمین کنگره ملی مهندسی شیمی ایران

تعداد صفحات: ۴

نویسنده(ها):

Yeghane – PhD Student in Chemical Engineering – Biotechnology
Shojaosadati – Professor in Industrial Biotechnology
Sadeghi zadeh – Associated Professor in Molecular Genetic
Khalilzadeh – Assistant Professor in Chemical Engineering – Biotechnology

چکیده:

Human interleukin-2 was expressed in Escherichia coli BL21(DE3) under the control of T7 promotor.Glucose as carbon and energy source was used with a simple feeding method based on maximum specific growth rate of recombinant E.coli. Cell density in culture was reached to 71±۲ g dry wt l _1 in 9 h after beginning of fed batch.