سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۳

نویسنده(ها):

S.H Hejazi – Parasitolofy and Mycology Department . School of Medicine, Isfahan University of Medical Sciences
S Jamali – Parasitolofy and Mycology Department . School of Medicine, Isfahan University of Medical Sciences
P Nasirfar – Parasitolofy and Mycology Department . School of Medicine, Isfahan University of Medical Sciences
P Pournaderi – Parasitolofy and Mycology Department . School of Medicine, Isfahan University of Medical Sciences

چکیده:

Iran is endemic for both zoonotic Leishmaniasis (ZCL) caused by Leishmania major and anthroponotic cutaneous leishmaniasis (ACL) caused by L. tropica. The ancient city of Isfahan is sespected to harbour endemic foci for both ZCL and ACL. Control measure require thorough knowledge of Leishmania ecology and epidemiology. The methods for Leishmania species detection andidentification become crucial. In this study the PCR method with species specific primers was used to identify Leishmania species causing CL In these foci. Thirty three samples were taken from parasitologically proven CL patients. The samples were cultured and then harvested promastigotes were washed 3 times and amplified kDNA products were analuzed by electrophoresis in 2% agarose gel. The gel was stained with ethidium bromide. Among 33 isolates investigated 28 isolates were identified as L major and 5 isolates were identified as L. tropica. The results concluded that PCR is a crucial method for identification of Leishmania spp, and that Isfahan is endemic for both ZCL and ACL, although the majority of cases being ZCL.
This study was supported partially by TDR/WHO and partially by Center for Research and Training in Skin Disease and Leprosy.