سال انتشار: ۱۳۸۷

محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان

تعداد صفحات: ۱

نویسنده(ها):

Z Sattari – School of Veterinary Medicine, Shahrekord University, Iran.
H Malekian –
A Nematollahi –
S Habibian Dehkordi –

چکیده:

Objective: Flavobacterium psychrophilum, the causal agent of bacterial cold water disease (BCWD) and rainbow trout fry syndrome (RTFS), has been recognized as a world-wide occurring pathogen in fresh water aquaculture. Infections of farmed salmonids by this bacterium represent major cause of economic losses and fish mortality in fish farming industry in many countries. This study aims at defining the effect of ion composition of water and temperature on the adhesion and colonization of F. psychrophilum with gills, by using an in vitro gill perfusion model.
Method & Materials: The ability of F. psychrophilum to attach to the gills of Rainbow Trout (Oncorhynchus mykiss), was studied using a gill perfusion model. A comparison was made between a high and a low virulence strain of F. psychrophilum and evaluated in comparison to results obtained previously with an in vivo model. The ion composition of the water of the organ bath in which the gills were suspended was varied and the influence on adhesion processes assessed. Experiments were carried out to examine the influence of water quality and temperature on the capacity of the bacteria to adhere.
Results & Conclusion: It was found that the high virulence strain adhered more readily than the low virulence strain, as was found during the in vivo
experiments. Moreover, it was observed that adhesion of the high virulence strain was enhanced by a number of factors. These were immersion of the gill in bivalent, ion-rich water, the presence of nitrite or organic matter, and high temperatures.