سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۵

نویسنده(ها):

Vahid Karimi – Faculty of Veterinary Medicine, University of Tehran
Mohammad Hassan Bozorgmehri Fard – Faculty of Veterinary Medicine, University of Tehran
Delavar Shahbazzadeh – Dept. of Biotechnology, the Pasteur Institute of Iran,Tehran
Majid Esmaelizad – Dept.of Biotechnology, Razi Vaccine and Serum Research Institute, Karaj

چکیده:

Sequence analysis and phylogenetic study of hemagglutinin (HA) gene of H9N2 subtype of avian influenza virus isolates (outbreaks of 1998-2002) in Tehran province (Iran) were studied. PCR products of a 430-bp fragment of 16 isolates were sequenced and then were aligned with the reported sequences in Genbank. Nucleotide sequence comparisons of HA gene from Iranian isolates showed 97-99% identity within the group, and 98% homology with the two isolates [A/Parakeet/Narita/92A/98 (H9N2)] and [A/Parakeet/Chiba/1/97 (H9N2)] from Pakistani parakeets imported to Japan. On the basis of phylogenetic evidence and even sequence comparison of Neuraminidase (N) gene of [A/Parakeet/Chiba/1/97 (H9N2)] and [A/Chicken/Iran/IT/99 (H9N2)](Li,2002) it is proposed that the emergence of H9N2 avian influenza infection in Iran originated in Pakistan. Due to the high percentage of H9N2 homology isolates of Iran with other isolates, namley A/quail/HongKong/G1, in Genbank and based on published reports for high similarity with infecting human H5N1 isolates, it seems that the potential of Iranian avian influenza isolates to infect human should be considered