سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۳

نویسنده(ها):

H Moravej – Dermatology Department, Faculty of Medical Sciences, Shahid Beheshti University, Tehran, Iran
Abbas Doosti – Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran & Islamic Azad University- Shahrekord Branch
A Vessal – Dermatology Department, Faculty of Medical Sciences, Shahid Beheshti University, Tehran, Iran
Hosseinzadeh – Dermatology Department, Faculty of Medical Sciences, Shahid Beheshti University, Tehran, Iran

چکیده:

To investigate Leishmania Sp. DNA in cutaneous paraffin embedded blocks, a hemi-nested PCR method was developed and tested five cutaneous paraffin blokes which suspected to Leishmania Sp. infection. DNA form paraffin blocks were extracted by DNP™ DNA Extraction kit and then subjected for PCR and sequencing. An internal control was carried out that determined human factor XIII genome to investigate accuracy of DNA extraction. Three of five samples showed positive Leishmania DNA amplification. Results of sequencing showed the Leishmania genome sequences in all three positive samples. In conclusion, the developed hemi-nested PCR method can readily detect Leishmania DNA in the paraffin embedded samples that are suspected to be infected with Leishmania.