دانلود مقاله Molecular investigation of viral nervous necrosis in Acipenser persicus from Caspian Sea
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
S Haghighi Karsidani – International Sturgeon Research Institute
S.J Zorriehzahra – Coldwater Fish Research Centre, Tonekabon, Iran
M Pourkazemi – International Sturgeon Research Institute
A Shenavar Masouleh – International Sturgeon Research Institute
Method & Materials: We examined 87 specimens on brain, eye, sperm and egg from male and female brood stocks that were taken from southern coast of Caspian Sea during spring and summer in 2006 and 2007. In this study, we use IQ2000 RT-PCR kit. RNA extraction was carried out using RNA extraction solution included in the kit based on phenol-chloroform method. RNA qulity was checked by electrophoresis of RNA on 1-2% agarose gel. Target segment of RNA was amplified by RT-PCR and Nested-PCR based on protocol of IQ2000 kit.
Results & Conclusion: Consequences of Nested-PCR were electrophoresed on 2% agarose gel and was stained by Ethidium Bromide. Based on protocol of kit, negative samples indicate a sharp band on 630 bp. In this study all of samples displayed bands with 630 bp, so all of samples were negative.