سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۴

نویسنده(ها):

Shahriar Osfouri – Department of Chemical Engineering, Persian Gulf University, Boushehr.
Pasquale Stano – Dipartimento di Biologia; Università degli Studi di Roma Tre; Viale Marconi.
Pier Luigi Luisi – Dipartimento di Biologia; Università degli Studi di Roma Tre; Viale Marconi.

چکیده:

DNA was studied in lipid reverse micelles with the aim of investigating the interactions DNA/lipid in a restrictedcompartment with minimal water content. Circular dichroic (CD) spectra of DNA at low water content showed the characteristic polymer salt induced (psi) spectra of condensed DNA. Dynamic light scattering (DLS) showed a peak around 400 nm radius (DNA-containing micelles), and a peak around 2.5 nm (“empty” micelles). In addition, CD and DLS results were rather well reproducible and considerable time stability.