سال انتشار: ۱۳۸۷

محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان

تعداد صفحات: ۱

نویسنده(ها):

Andrzej Siwick – Department Fish Pathology and Immunology, IFI in Olsztyn, Poland
Krzysztof Kazuń –
Barbara Kazuń –
Elżbieta Terech-Majewska –

چکیده:

Objective: The iridoviruses contain large double-stranded DNA viruses found both among invertebrate and vertebrate hosts. The clinical signs and histopatological analyses suggested that haematopoietic tissues are particularly susceptible to fish iridoviruses. Actually, we have a very few information about effect of iridovirus on the cell-mediated and humoral mediated immunity. Preliminary study suggested that iridoviruses pathogenic on the sturgeon and catfish increases nonspecific cellular and humoral defence mechanisms.
Method & Materials: In our study we examined the influence of iridovirus (59.90, LDA-39, France) on the macrophages and lymphocytes activity isolated from spleen and head kidney. Also we examined the influence of iridovirus on the lysozyme and ceruloplasmine activity in serum. For this study we used 200 healthy European catfish. Fish were maintained in two 500 l tanks at temperature 22°C. Experimental group was infected by intraperitoneal injection of iridovirus. The blood and organs were separated from 10 fish of experimental and control group before and 1, 3, 5, 7 and 14 days after infection.
Results & Conclusion:
The results showed that iridovirus (59.90) significantly (P<0.05) decreased the phagocytic ability of spleen macrophages and proliferative responses of head kidney T and B cells induced by mitogens. Also this virus statistically decreased lysozyme activity and increased ceruloplasmine activity in serum. It seems that one influence of the early-induced iridovirus pathogenicity in European catfish is the inhibition of the cell-mediated immunity and lysozyme production by leucocytes and increased acute phase of protein in hepatocytes presented by ceruloplasmine activity.