دانلود مقاله Pathogenecity of iridovirus – influence on innate immunity in European catfish (Silurus glanis)
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
Andrzej Siwick – Department Fish Pathology and Immunology, IFI in Olsztyn, Poland
Krzysztof Kazuń –
Barbara Kazuń –
Elżbieta Terech-Majewska –
Method & Materials: In our study we examined the influence of iridovirus (59.90, LDA-39, France) on the macrophages and lymphocytes activity isolated from spleen and head kidney. Also we examined the influence of iridovirus on the lysozyme and ceruloplasmine activity in serum. For this study we used 200 healthy European catfish. Fish were maintained in two 500 l tanks at temperature 22°C. Experimental group was infected by intraperitoneal injection of iridovirus. The blood and organs were separated from 10 fish of experimental and control group before and 1, 3, 5, 7 and 14 days after infection.
Results & Conclusion:
The results showed that iridovirus (59.90) significantly (P<0.05) decreased the phagocytic ability of spleen macrophages and proliferative responses of head kidney T and B cells induced by mitogens. Also this virus statistically decreased lysozyme activity and increased ceruloplasmine activity in serum. It seems that one influence of the early-induced iridovirus pathogenicity in European catfish is the inhibition of the cell-mediated immunity and lysozyme production by leucocytes and increased acute phase of protein in hepatocytes presented by ceruloplasmine activity.