سال انتشار: ۱۳۸۷

محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان

تعداد صفحات: ۱

نویسنده(ها):

J. Szarek – University of Warmia and Mazury, Olsztyn, Poland
A Andrzejewska – Medical University, Bialystok, Poland
K Skibniewska – University of Warmia and Mazury, Olsztyn, Poland
J Guziur – University of Warmia and Mazury, Olsztyn, Poland

چکیده:

Objective: Different plant protection chemicals, including DDT, were intensively eliminated about 35 – 45 years ago in Europe. 350 pesticide tombs (PT) arose at that time in Poland. They became dangerous for the environment mainly from their bad technical condition and incorrect localization. The aim of this investigation was to study how the morphological pattern of the carp organs reflected environmental pollution by the PT.
Method & Materials: The research was carried out in the area of Ilawskie Lake District (Poland) near the PT (53.78 Mg plant protective agents, mainly DDT), incorrectly protected (2004) and 2, 3 and 4 years after it was closed down (2006 – 2008). The investigations were undertaken on 80 carps (85 – ۱۰۰ g: 40 – from the pond (1) situated 100 m from PT and 40 from the control one (2) localized on the not polluted area. The macroscopic microscopic (hepatopancreas and kidneys – hematoxylin and eosin staining, Sudan III (Lillie Ashburn method) and PAS according to McManus) and ultrastructural analyses (hepatopancreas) were carried out.
Results & Conclusion:
The study showed that the number of morphological lesions and the degree of their intensity were higher in hepatopancreas of the carps from pond 1 in comparison to fish from the pond 2. Although the differences between the number of particular lesions were not statistically important, their sum was. The biggest differences in this matter are visible among retrogressive changes of the analyzed organ in fish from the
pond 1 in comparison with control carps. Ultrastructurally, it was noted that there were not only destructive lesions in the hepatocytes of carps
from the pond 1 (damage of mitochondria, myelin-like structures, degradation of cytoplasmatic structures) but also there were adaptive lesions (mitochondrial proliferation and enlargement of the rough and smooth endoplasmatic reticulum canals). Hepatocytes of fish caught in the pond 2 did not show significant subcellular changes. Observed distribution and character of morphological lesions (particularly in hepatopancreas) in carp living in pond situated close to pesticide tomb in comparison with carp from the pond which was far from the source of DDT indicate that the reason is environmental pollution by PT. The investigation shows that after 4 years the PT was closed down it still (slightly) influences fish bred in close to pond.