سال انتشار: ۱۳۸۴

محل انتشار: سیزدهیمن کنفرانس مهندسی برق ایران

تعداد صفحات: ۶

نویسنده(ها):

Bahrami – University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO17 1BJ, United Kingdom
Melvin – University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO17 1BJ, United Kingdom
Wilkinson – University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO17 1BJ, United Kingdom
Evans – University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO17 1BJ, United Kingdom

چکیده:

In this paper, we present both a theoretical evaluation and the fabrication of a novel polymerase chain reaction (PCR) microdevice. This microdevice contains elements for both thermal cycling and fluorescence detection. The proposed device is composed of a reaction chamber with integrated temperature sensor, heaters, p-n diode and optical filter. The advantage of combining these in a single structure is that real time detection of DNA amplification will be possible using a small volume of the PCR solution. The photodiode is covered by a thin film optical filter in order to block out the light which is used to excite the fluorophore dyes. CdS is used for the first time for such a filter and the complete micro-fabrication process is described