سال انتشار: ۱۳۸۴

محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران

تعداد صفحات: ۱۰

نویسنده(ها):

Mojtaba Tabatabaei Yazdi – Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences
Gholamreza Zarrini – Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences
Elham Mohit – Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences
Mohammad Ali Faramarzi – Department of Phramaceutical Biotechnology, Faculty of Pharmacy, Tehran university of Medical Sciences

چکیده:

۱۶۵ Strains of microorganisms with ability to degrade uric were isolated from soil samples during a screeming program. Among them, a zygomucete fungus with well developed columellae showed to produce high levels of the enzyme in a short time. Isolated fungus was identified as Mucor hiemalis according to the morphological and culture characteristics of the organism. The fungus was able to produce an intracellular urate oxidase in a fermentation medium mainly containing uric acid. Optimized composition of the medium consisted of (1 of distilled water) uric acid, 7.0g, maltose, 6.0 g , Vogel stock solution, 20.0 ml and 1.0 ml of 0.5 M copper sulphate.The optimum pH and temperature for uricase production in the optimized medium were pH 6.0 and 30 C, respectively