دانلود مقاله Protection of juvenile Penaeus indicus from Vibrio parahaemolyticus infection by Bacillus subtilis
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
M Avakh Keysami – Technical and Vocational Higher Education Center of Jihad-Agriculture Ministry, Bushehr
M Soltani – Department of Aquatic Health, Faculty of Veterinary, University of Tehran, Iran
E Sharifpour – Department of Aquatic Healtht, Iranian Fisheries Research Institute
M Mohamadpour – Department of Research Management, Bushehr University of Medical Sciences, Iran
The objective of present study was to investigate the potential probiotic properties of Bacillus subtilis in Penaeus indicus. Method & Materials:
Bacillus subtilis bacterium isolated from intestine of P. indicus juveniles were added to pelleted feed as a probiotic at level 1010 cfu/g feed by soaking method. After 60 days feeding trial with Bacillus supplemented and non-supplemented control feeds, P. indicus from 0.429± ۰٫۰۱۴g exhibited significant differences (P<0.05), between Bacillus and control groups in growth and survival. Sixty days after the start of the Bacillus feeding, the prawns were challenged by bath exposure to Vibrio parahaemolyticus (107 cell/ ml), for 28 days.
Results & Conclusion:
Two weeks after challenging, cumulative mortality was significantly (P<0.05) reduced in probiotic treated group. Probiotic treated group had 88.33% survival rate; whereas the control group had only 20.83% survival. In addition, the control group had an unhealthy external appearance, while treated group prawn, appeared healthy and normal. From this, it was concluded that the selected Bacillus sp. may be a promising probiotic to protect P. indicus from infection with Vibrio parahaemolyticus.