سال انتشار: ۱۳۸۶

محل انتشار: پنجمین کنگره بین المللی مهندسی شیمی

تعداد صفحات: ۹

نویسنده(ها):

Farshad Rahimpour – Biotechnology Research lab, Chemical Engineering Department, Faculty of Engineering, Razi University, Kermanshah 67149-67346, Iran, Department of Biotechnology, Center of Chemistry and Chemical Engineering, Lund University,Lund, Sweden
Rajni Hatti-Kaul – Department of Biotechnology, Center of Chemistry and Chemical Engineering, Lund University, Lund, Sweden

چکیده:

A two-stage PEG- phosphate aqueous two-phase system was used for purification of extracellular recombinant xylanase. Influence of the different factors affecting the partitioning of recombinant xylanase and contaminant proteins, like pH, polyethylene glycol (PEG) molecular weight, PEG and phase forming salt concentrations and NaCl concentration were studied. After back-extraction step, specific activity of xylanase was increased 6.71 times, and the total enzyme yield of 78% was obtained.