سال انتشار: ۱۳۸۶
محل انتشار: پنجمین کنگره بین المللی مهندسی شیمی
تعداد صفحات: ۹
Farshad Rahimpour – Biotechnology Research Lab., Chemical Engineering Dept., Faculty of Engineering, Razi Univ., 67149-67346 Kermanshah, Iran,Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
Rajni Hatti-Kaul – Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, Lund, Sweden
Farzaneh Feyzi – Chemical Engineering Faculty, Iran University of Science and Technology, Narmak, Tehran, Iran
The influence of pH, salt concentration, polyethylene glycol (PEG) molecular weight and concentration, enzyme loads and temperature on the enzyme refolding has been evaluated. To optimize and build the mathematical model and minimize the number of experiments for the design parameters, response surface methodology with a face-centered central composite design (CCF) was defined. The adequacy of the calculated model for the response was confirmed by means of variance analysis and additional experiments. Recovery of more than 99% active xylanase was obtained for a system with 18.3% (w/w) PEG 1000, 14.4% (w/w) phosphate at pH 8.5, and enzyme load corresponding to a protein concentration of about 0.05 mg/g system. The optimum temperature was found to be 22 º C.