سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
R Hobbenaghi – Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
A Tukmechi – Department of Pathophysiology and Quality Control, Artemia and Aquatic Animal Research Institute, Urmia University, Urmia, Iran.
H Rahmati-Holasoo – Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
A Morvaridi – Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Method & Materials: In September 2007 a 2.5-year-old male oscar with clinical signs of a small pink nodule on right side of its lower jaw, two
similar nodule on the ventral surface of lower jaw and in the mouth with a gradual increase in the nodule size was delivered to the laboratory of pathology of Veterinary College, Urmia University. The fish deceased and the samples were taken from nodules and surrounding tissues for pathological analysis. The tissue samples were fixed in 10% formalin and embedded in paraffin wax. Sections were prepared and stained with Haematoxylin and Eosin and Ayoub-Shklar methods.
Results & Conclusion: Histopathological analysis of the sections revealed the presence of multiple cell masses under skin layers. There were necrotic and degenerated red-colored cells with picnotic nucleus or without nucleus like keratin in the central part of these cell masses. The invaded cell masses to dermis had some neoplastic changes including pleomorphism in cells and nucleus. For diagnosis confirmation special staining procedure of Ayoub-Shklar was performed. Accordingly, the presence of keratin in the central part of invaded cell masses into dermis was uncovered. Indeed, red-colored keratin mass (cancer pearl) has been in a bluish background of tissue component.