سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۳
Rezvan Esmaeili – Biotechnology Research Center, Pasture Institute of Iran
Ramin Sarrami Forooshani –
Ahmad Adeli –
Samad Amini –
Quantification of hepatitis B virus (HBV) DNA in serum is important for monitoring treatment. We tried to develop a quantitative assay system for HBV DNA based on taqman real-time quantitative polymerase chain reaction it was done in the iCycler™(Bio Rad) apparatus. Primers and a probe for sequences of the surface gene of HBV were designed and quantification accomplished by reference to standards containing known concentrations of the target sequence. Different concentration of MgCl2 was tested to optimize the reaction. This assay is able to quantify HBV DNA from 109 to 104 copies/reaction. Seven HBV positive sera and serial dilutions of the Iranian patients were analyzed. The assay detected all HBV positive samples.