سال انتشار: ۱۳۸۷

محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان

تعداد صفحات: ۱

نویسنده(ها):

F Heidary Jamebozorgi – Faculty of Natural Resources and Marine Sciences, Tarbiat Modarres University
I Sharifpour –
B Abtahi –
S.J Seyfabadi –

چکیده:

Objective: The water-soluble fraction (WSF) of crude oil is a highly volatile complex and toxic mixture of hydrocarbon chains (polyaromatics, heterocyclics), phenols, and heterocyclic compounds containing nitrogen and sulfur. Histopathological effect of WSF of crude oil, in different concentrations, on vital organ, gills, of juvenile Rutilus frisii kutum, Cyprinidae, an endemic fish of the Caspian Sea, was investigated.
Method & Materials: Juvenile specimens weighting approximately 1 g were acclimated to the laboratory conditions for a minimum of 8 days in a 20- L glass aquarium with dechlorinated water. Prior to the definitive experiment, the phase of the total dissolved WSF in water during 96 h was prepared by using two different concentrations (0.1 and 0.5 of LC50) of crude oil WSF. After 24, 48, and 96 h of exposure, 3 individuals for each of the two WSF doses (0.1 and 0.5 of LC50) and three controls (one for 24, 48 and the other for 96 h) were collected.
Results & Conclusion: Pathological changes in the gills included, congestion in primary and secondary lamellae, clubbing, fusion, sever edema in basal membrane of secondary lamellae, hypertrophy of epithelial cells of secondary lamellae, presence of melanin pigments in the gill, initiation of hyperplasia in between secondary lamella and decreasing in the size of secondary lamella was observed. This study demonstrated that the WSF of crude oil causes serious damage in gills of juvenile Rutilus frisii kutum and also provided the basis for a better understanding of the toxic mechanisms of WSF in freshwater fishes.