سال انتشار: ۱۳۸۴
محل انتشار: چهارمین همایش ملی بیوتکنولوژی ایران
تعداد صفحات: ۴
A Salehzadeh – 1Biology Dept., Faculty of Science, Razi Univ., Kermanshah, Iran
M Motallebi – National Istitiute for Genetic Engineering & Biotechnology (NIGEB), Tehran, Iran.
M.R Zamani – National Istitiute for Genetic Engineering & Biotechnology (NIGEB), Tehran, Iran.
In this research to study the inhibiting effect of polygalacturonase inhibiting protein(PGIP) on polygalacturonase(PG) enzyme from Fusarium oxysporum f.sp lycopersici, (highly virulent isolates), protein extraction was carried out from various tissues (stem, leaf and root) of 40,60 and 80 days old tomato plants(lycopersicon esculentum c.v. Star). Study of PGIP- PG interaction showed correlation between the plant age and increasing effect of inhibition activity of PGIP with higher effect of stem PGIP. There were some differences in protein patterns of induced and non-induced tomato plants by F. oxysporum. Tomato PGIP showed different inhibitory activity on PG extracted from different phytopathogenic isolates of F. oxysporum. Also, for pgip gene detection in tomato plant, an expected 1174 bp fragment was amplified and confirmed by HindIII and EcoRI restriction enzymes.