دانلود مقاله Using pathogenicity challenge test for Assessment of putative Bacillus subtilis as probiotic in juvenile P. indicus
سال انتشار: ۱۳۸۷
محل انتشار: اولین کنگره بین المللی مدیریت بهداشتی و بیماریهای آبزیان
تعداد صفحات: ۱
M Avakh Keysami – Technical and Vocational Higher Education Center of Jihad-Agriculture Ministry, Bushehr, IRIran
M Soltani – Department of Aquatic Health, Faculty of Veterinary, University of Tehran, IRIran
E Sharifpour – Department of Aquatic Health Management, IFRO, IRIran
M Mohamadpour – Department of Research Management, Bushehr University of Medical Sciences, IRIran
Method & Materials: In-vitro challenge test was carried out on juvenile shrimps (0.1g) using the immersion method. An in-vivo challenge experiment
was carried out using the feeding trial method. Bacterial suspension was added to the challenge feed at a concentration of 1010 cells/ ml, except in the control diet. Survival rate during four weeks post challenge was determined weekly.
Results & Conclusion: The result of in-vitro pathogenicity tests indicated that exposure to Vibrio parahaemolyticus, induced significant (p<0.05)
mortalities in P. indicus within 12 hours at 107 cells/ml and 24 h at 105 cells/ml level. In in-vivo study the survival of juvenile (0.45g) was not affected by the presence of Bacillus in fresh water but, V. parahaemolyticus infected juvenile P. indicus and cause mortality about 78.67% and exhibited disease clinical signs. There were significant differences among control and Bacillus sp tanks with V. parahaemolyticus tanks on the prawn survival (P < 0.05). From this, it was concluded that the putative Bacillus subtilis may be a promising probiotic for juvenile P. indicus.